• Philosophy

Rabbit immune system is an unrivaled system for generating antibody diversity

Rabbit antibodies are known for their excellent affinities and specificities; even generating antibodies to unique epitopes. The explanation for these attributes and the broad diversity of antibodies are the mechanisms employed by the rabbit immune system [read more].

Large diversity requires high throughput technologies

In order to yield the most diverse repertoire of target specific rabbit antibodies, and to allow for a fast identification of the monoclonal antibody with the best functional profile, as many of the IgG expressing B-cell clones as possible are selected and screened in an automated process. 15,000 to 60,000 B-cell clones are isolated and cultured individually per campaign. The secreted monoclonal antibodies can be functionally tested in high throughput assays.

Sequence diversity

After sequencing thousands of target-specific antibody genes, it is remarkable that we have never isolated the same sequence from different rabbits immunized with the same immunogen. This supports the assumption that gene conversion produces individual rabbits with a unique gene repertoire for antibodies. Also, the sequence redundancy rate in one individual animal is very low. This is the result of the empirically optimized immunization and sampling protocol. We can guarantee novel sequences from each campaign, a solid basis for IP [read more].

Fc isotype diversity

Function, efficacy and safety profile of therapeutic antibodies can be highly dependent on the antibody isotypes and Fc modifications. Therefore, we introduced special features to allow for flexibility in targeted modification of the antibody’s Fc part [read more].

Outcome: Unique antibodies with diverse functionality

Altogether, the above-mentioned factors and procedures result in sets of functional antibodies with unique and diverse functional profiles [read more].